terminal transferase
英
美
网络 末端转移酶; 末端脱氧核苷酸转移酶; 末端核苷酸转移酶; 末端标记或加尾法
双语例句
- Estimation of Postmortem Intervals by Using Terminal Deoxynucleotidyl Transferase; Partial Inhition of DNA Polymerase and Terminal Deoxyribonucleotide Transferase Activities from Bovine Thymus Lymphocytes
应用DNA3末端转移推测死亡时间胸腺淋巴细胞末端脱氧核苷酸转移酶和脱氧核糖核酸α-聚合酶活性的部分抑制 - The morphological change, DNA breakage and the change of DNA content of the apoptotic cells in different cell cycles were detected by Transmission electronic microscope, Terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL) assay and flow cytometry.
以透射电子显微镜、末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）法和流式细胞术分别检测调亡细胞的形态学变化、DNA断裂情况和不同细胞周期的DNA含量变化。 - Apoptotic cardiomyocytes were detected using the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling method ( TUNEL). The expression of BAX and BCL-2 proteins was determined by immunohistochemical method.
用原位末端标记法（TUNEL）标记凋亡的心肌细胞，用免疫组织化学方法检测BAX和BCL-2的蛋白表达。 - Methods Semisolid culture, DNA agarose electrophoresis, in situ terminal deoxynucleotidyl transferase assays, view with electromicroscope.
方法骨髓细胞半固体培养和悬浮培养，DNA凝胶电泳，DNA缺口末端标记（TdT），电镜观察。 - We observed apoptosis with terminal transferase deoxyuridine nick-end assays and tested NO of the prostate tissue with nitrite reduction test then calculated the activity of NOS.
通过原位末端检测技术测定前列腺组织细胞凋亡情况；应用亚硝酸盐还原酶法检测前列腺组织中NO变化并计算NOS活力的变化； - Methods: The 30 bp-sequence was synthesized and labeled with digoxigenin by terminal transferase.
方法：人工合成30bp负调控序列，用末端转移酶对其进行地高辛标记； - Purification and characterization of the terminal deoxynucleotidyl transferase from pig thymus
猪胸腺末端脱氧核苷酰转移酶的分离纯化及性质的研究 - Combined with automatic imaging analysis technique, cardiac myocytes 'apoptosis in left ventricle was detected by using in situ terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL) staining.
终未脱氧核糖核苷酸转移酶介导的原位缺口末端标记（TUNEL）法结合图像分析技术检测左室心肌细胞凋亡； - The apoptosis of mice melanoma B16 cells induced by β-elemene was studied with the electron microscope and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL) technique.
目的研究β－榄香烯（β－E）诱导小鼠黑色素瘤B16细胞发生凋亡的作用。方法应用透射电镜和TdT介导的脱氧核苷酸缺口末端标记（TUNEL）技术。 - OBJECTIVE: To investigate RAG gene, DNA repair factors Ku70/ Ku80 and terminal deoxynucleotidyl transferase ( TdT) mRNA expression and T cell receptor ( TCR) gene recombination in human leukemia and lymphoma cell lines.
目的：检测重组激活基因、DNA修复因子Ku70/Ku80和末端脱氧核苷转移酶mRNA表达以及T细胞受体基因重排在人白血病和淋巴瘤细胞株的发生情况。 - DNA polymerase I mediated biotin dATP nick translation ( PANT) and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL) were adopted for in situ identification of single and double strand DNA fragmentation, respectively.
采用原位PANT（DNA聚合酶I介导的生物素标记的dATP缺口平移）及原位TUNEL（末端脱氧核苷酸转移酶介导的dUTP末端标记），分别检测DNA损伤的单链断裂及双链断裂。 - Using terminal deoxynucleotidyl transferase-mediaed dUTP nick end labeling ( TUNEL), apoptotic cells were detected in 80 specimens of colorectal carcinoma.
采用脱氧核糖核酸末端转移酶介导的缺口末端标记技术（TUNEL），对80例结直肠癌组织中凋亡的TIL及肿瘤细胞进行观察。 - VSMC apoptosis was measured by terminal deoxynucleotidyl transferase biotin nick end labeling ( TUNEL).
采用原位缺口末端标记方法（TUNEL）检测大鼠肺动脉平滑肌细胞的凋亡，并行图像分析计算单位面积细胞凋亡率； - METHODS Cytotoxicity assay was tested by MTT method. The influence of neferine to affect vincristine to induce gastric carcinoma apoptosis was detected by PI staining flow cytometry, AO/ EB double fluorescence stain and terminal deoxynucleotidyl transferase mediated nick end labeling ( TUNEL).
方法采用MTT比色法检测药物的细胞毒性作用，并用流式细胞术、AO/EB双重荧光染色法和TUNEL法测定甲基莲心碱对长春新碱诱导人胃癌细胞凋亡率的影响。 - Evidence also shows that DNA polymerase mu ( pol μ) is only present in mammals, and is a duplicated gene of terminal deoxynucleotidyl transferase ( TdT).
DNA聚合酶mu（polμ）基因仅存在于哺乳动物基因组中，是脱氧核苷酸末端转移酶（TdT）的重复基因； - After developing a zinc deficient rat model, using TUNEL ( terminal deoxynucleotidyl transferase mediated dUTP nick endlabeling) method, the apoptosis of thymocytes and spleen lymphocytes was checked.
【方法】建立生长期缺锌大鼠模型，对免疫器官胸腺、脾脏进行大体观察，并用原位末端标记（TUNEL）方法，利用图像分析系统对胸腺和脾脏淋巴细胞的凋亡进行定性和半定量研究。 - Apoptosis was observed using terminal transferase marking method.
末端转移酶标记法原位检测大脑神经细胞凋亡状况。 - Cardiomyocyte apoptosis was detected with In situ terminal deoxynucleotidyl transferase ( TdT)-dUTP nick-end labeling ( TUNEL staining) and DNA gel electrophoresis.
末端脱氧核糖核酸转移酶介导的dUTP切口末端标记技术（TUNEL）和DNA凝胶电泳检测心肌细胞凋亡。 - The effect of thymic hormone on terminal deoxynucleotidyl transferase
胸腺激素对末端脱氧核苷酸转移酶的影响 - In situ terminal deoxynucleotidyl transferase ( TdT) labeling ( TUNEL) and DNA ladder were applied to detect apoptosis.
运用原位末端脱氧核苷转换酶（TdT）标记法（TUNEL）和DNA阶梯法检测细胞凋亡。 - Methods: Apoptotic cells were detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL) in 34 BMSC cases;
方法：采用末端脱氧核苷酰转移酶介导的dUTP缺口末端标记（TUNEL）法检测凋亡细胞，计算凋亡指数。 - The express of TUNEL positive cells by terminal deoxynucleotidyl transferase mediated dUTP nick end-labelling;
TdT介导的dUTP缺口末端标记法检测TUNEL阳性细胞表达； - After radiation for 12 hours, the apoptosis in hippocampal dentate gyrus was detected with the cutting end labeling method mediated by terminal deoxynucleotidyl transferase.
照射后12h，用末端脱氧核苷酸转移酶介导的切口末端标记法检测海马齿状回凋亡细胞； - Rods, cones, and apoptotic cells were labeled by antibodies of anti-rod and anti-cone cells, and terminal deoxynucleotidyl transferase dUTP nick-end labeling ( TUNEL), respectively.
抗视锥和抗视杆细胞的抗体分别标记视锥和视杆细胞，dUTP缺口末段标记法（TUNEL）标记凋亡细胞。 - Methods Using immunocytochemistry ( ICC), terminal deoxynucleotidyl transferase mediated end labeling ( TUNEL) and ICC/ TUNEL double label methods, the expression of PAI 2 in and the apoptosis of the cultured epidermal keratinocytes treated by TNF α were detected.
方法利用免疫细胞化学、TUNEL反应及免疫细胞化学TUNEL双标等方法，观察TNFα作用下，培养的表皮角质形成细胞及其脱落细胞中PAI2表达的变化及凋亡发生的情况。 - Eyeballs were selected separately, retinals digesting stretched preparation capillary samples were prepared, and apoptosis cells were specificity marked by PAS dyeing and ribonucleotide terminal transferase mediating dUTP nick-translation ( TUNEL method), to observe below light microscope.
分别按期摘取眼球，制备视网膜消化铺片微血管标本，PAS染色及核苷酸末端转移酶介导dUTP缺口翻译法（TUNEL法）特异性标记凋亡细胞，光镜下观察。